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Addgene

pESF140_HisSpy_DDB1∆B
(Plasmid #124213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC8
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA damage-binding protein 1
  • Alt name
    DDB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2508
  • Mutation
    internal deletion of BPB domain to aid crystallizationb
  • GenBank ID
    NM_001923.4 NM_001923.4
  • Entrez Gene
    DDB1 (a.k.a. DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF)
  • Tag / Fusion Protein
    • His6-Spy (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESF140_HisSpy_DDB1∆B was a gift from Eric Fischer (Addgene plasmid # 124213 ; http://n2t.net/addgene:124213 ; RRID:Addgene_124213)

  • For your References section:

    Plasticity in binding confers selectivity in ligand-induced protein degradation. Nowak RP, DeAngelo SL, Buckley D, He Z, Donovan KA, An J, Safaee N, Jedrychowski MP, Ponthier CM, Ishoey M, Zhang T, Mancias JD, Gray NS, Bradner JE, Fischer ES. Nat Chem Biol. 2018 Jul;14(7):706-714. doi: 10.1038/s41589-018-0055-y. Epub 2018 Jun 11. 10.1038/s41589-018-0055-y PubMed 29892083
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