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pBABE (hygro) mEOS2 hYAP 1-2alpha
(Plasmid #124159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE (hygro)
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEOS2-YAP1
  • Species
    H. sapiens (human)
  • Mutation
    None (Wild Type)
  • GenBank ID
    NP_001181973
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site YAP cloned EcoRI-SalI immediately 3' to mEOS2 (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert size is approximately 3530. The insert is a fusion of mEOS2 and YAP1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE (hygro) mEOS2 hYAP 1-2alpha was a gift from Megan Finch-Edmondson & Marius Sudol (Addgene plasmid # 124159 ; http://n2t.net/addgene:124159 ; RRID:Addgene_124159)

  • For your References section:

    Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene. Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M. Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. Epub 2012 Aug 24. 10.1016/j.gene.2012.08.025 PubMed 22939869
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