pcDNA3.1 (-) hYAP 1-2delta
(Plasmid
#124146)
-
PurposeProtein expression for funtional studies of cell growth promotion
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 124146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1 (-)
-
Vector typeUnspecified
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameYAP1
-
SpeciesH. sapiens (human)
-
MutationNone (Wild Type)
-
GenBank IDNP_001269030
-
Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert size is approximately 1580.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1 (-) hYAP 1-2delta was a gift from Megan Finch-Edmondson & Marius Sudol (Addgene plasmid # 124146 ; http://n2t.net/addgene:124146 ; RRID:Addgene_124146) -
For your References section:
Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene. Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M. Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. Epub 2012 Aug 24. 10.1016/j.gene.2012.08.025 PubMed 22939869