pQE30-His-T7RNAP
(Plasmid #124138)

• Purpose: Bacterial expression plasmid for PURE system components
• Depositing Labs: Sebastian Maerkl, Takuya Ueda
• Publication: Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.

Backbone

• Vector backbone: pQE30
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable ; JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T7 RNA polymerase
• Alt name: T7 RNAP
• Species: Enterobacteria phage T7
• Entrez Gene: T7p07 (a.k.a. T7p07)
• Tag / Fusion Protein:
  • 6x-His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pQE30-His-T7RNAP.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is provided in NEB Stable cells from Addgene; however, the depositor originally cloned this plasmid in JM109 cells. It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.