pET29b-IPP1-His
(Plasmid #124137)

• Purpose: Bacterial expression plasmid for PURE system components
• Depositing Labs: Sebastian Maerkl, Takuya Ueda
• Publication: Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.

Backbone

• Vector backbone: pET29b(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inorganic pyrophosphatase
• Alt name: IPP1
• Alt name: PPase
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: IPP1 (a.k.a. YBR011C, PPA1)
• Tag / Fusion Protein:
  • 6x-His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pET29b-IPP1-His.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.