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Addgene

pET21a-TrpRS-His
(Plasmid #124120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tryptophanyl-tRNA ligase
  • Alt name
    trpS
  • Alt name
    TrpRS
  • Species
    Escherichia coli
  • Entrez Gene
    trpS (a.k.a. b3384, ECK3371, JW3347)
  • Tag / Fusion Protein
    • 6x-His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-TrpRS-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124120 ; http://n2t.net/addgene:124120 ; RRID:Addgene_124120)

  • For your References section:

    Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
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