pBS hTSP2 clone M1-B
(Plasmid
#12412)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescriptII KS+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM1-B TSP2
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Alt nameTSP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1334
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MutationFragment from Heparin Binding domain and Type 1 repeats of TSP2
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Entrez GeneTHBS2 (a.k.a. TSP2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS hTSP2 clone M1-B was a gift from Paul Bornstein (Addgene plasmid # 12412 ; http://n2t.net/addgene:12412 ; RRID:Addgene_12412)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/98/41/761147fc-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)