pAAV-UbC-mNeonGreen
(Plasmid #124102)

• Purpose: Expression of the fluorescent protein mNeonGreen in mammalian cells
• Depositing Lab: Pantelis Tsoulfas
• Publication: AAV expression plasmids for production of mNeonGreen (unpublished)

Backbone

• Vector backbone: pAAV-UbC
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size w/o insert (bp): 5162
• Total vector size (bp): 5897
• Modifications to backbone: The anti-luciferase cassette was deleted and eGFP was replaced with mNeonGreen
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen
• Species: Synthetic; Branchiostoma lanceolatum
• Insert Size (bp): 735
• Mutation: Codon usage was optimized for Homo Sapiens
• GenBank ID: KC295282.1
• Promoter: UbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: Common sequencing primers pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gene was synthesized to match the Human codon usage.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry