Skip to main content
Addgene

pEW65: pQE-81L H3(K79M)
(Plasmid #124100)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pQE-81L
  • Backbone size w/o insert (bp) 4663
  • Total vector size (bp) 5074
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Histone H3
  • Species
    X. laevis (frog)
  • Mutation
    K79M
  • Entrez Gene
    LOC108704296
  • Promoter T5

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCATAAAAAATTTATTTGCTTTGTGAGCGG
  • 3′ sequencing primer ATTCTCACCAATAAAAAACGCCCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEW65: pQE-81L H3(K79M) was a gift from Cynthia Wolberger (Addgene plasmid # 124100 ; http://n2t.net/addgene:124100 ; RRID:Addgene_124100)

  • For your References section:

    Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L. Worden EJ, Hoffmann NA, Hicks CW, Wolberger C. Cell. 2019 Feb 8. pii: S0092-8674(19)30151-5. doi: 10.1016/j.cell.2019.02.002. 10.1016/j.cell.2019.02.002 PubMed 30765112
Related items:
Commonly requested with: