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Addgene

6107 pETH6KT218
(Plasmid #1241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp104 (K218T)
  • Alt name
    Hsp104
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    K218T
  • Entrez Gene
    HSP104 (a.k.a. YLL026W)
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nde1 (not destroyed)
  • 3′ cloning site sac1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6107 pETH6KT218 was a gift from Susan Lindquist (Addgene plasmid # 1241 ; http://n2t.net/addgene:1241 ; RRID:Addgene_1241)

  • For your References section:

    Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter J, Lindquist S. Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. 10.1126/science.1098007 PubMed 15155912
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