pEW4: pET-32a Dot1L(2-386)
(Plasmid
#124099)
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PurposeDot1L(2-386) expression plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-32a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5888
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDot1L(2-386)
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SpeciesH. sapiens (human)
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Insert Size (bp)1155
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Entrez GeneDOT1L (a.k.a. DOT1, KMT4)
- Promoter T7
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Tag
/ Fusion Protein
- Trx-His-TEV (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid # 74173
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEW4: pET-32a Dot1L(2-386) was a gift from Cynthia Wolberger (Addgene plasmid # 124099 ; http://n2t.net/addgene:124099 ; RRID:Addgene_124099) -
For your References section:
Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L. Worden EJ, Hoffmann NA, Hicks CW, Wolberger C. Cell. 2019 Feb 8. pii: S0092-8674(19)30151-5. doi: 10.1016/j.cell.2019.02.002. 10.1016/j.cell.2019.02.002 PubMed 30765112
