pcDNA3.1-Orange_CaMBI_300
(Plasmid #124095)

• Purpose: orange calcium-modulated bioluminescent indicator, 300nM
• Depositing Lab: Michael Lin
• Publication: Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 10.1038/s41589-019-0256-z.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6330
• Total vector size (bp): 8751
• Modifications to backbone: Chicken beta-actin promoter + Chicken beta-actin intron+ rabbit beta-globin intron inserted between CMV enhancer and inserted gene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Orange CaMBI 300
• Species: Synthetic
• Insert Size (bp): 2421

Resource Information

• Supplemental Documents:
  • pcdna3-1-orange_cambi_300.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor sequence for this section of the plasmid.