pcDNA3.1-Orange_CaMBI_110
(Plasmid #124094)

• Purpose: orange calcium-modulated bioluminescent indicator, 110nM
• Depositing Lab: Michael Lin
• Publication: Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 10.1038/s41589-019-0256-z.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6330
• Total vector size (bp): 8760
• Modifications to backbone: Chicken beta-actin promoter + Chicken beta-actin intron+ rabbit beta-globin intron inserted between CMV enhancer and inserted gene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Orange CaMBI 110
• Species: Synthetic
• Insert Size (bp): 2430

Resource Information

• Supplemental Documents:
  • pcDNA3.1-Orange_CaMBI_110
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C-terminal copy of the CyOFP coding sequence is codon diversified to facilitate molecular biology procedures on Orange CaMBI and reduce the possibility of recombination between the two copies of the CyOFP coding sequence.