pLenti6.3_DTX1
(Plasmid
#124080)
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PurposeDTX1 with normal stop codon, 3 silent SNPs (1233) C>T, (1251) A>G, (1425) C>T)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6.3
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 7692
- Total vector size (bp) 9555
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDTX1
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SpeciesH. sapiens (human)
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Insert Size (bp)1863
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Entrez GeneDTX1 (a.k.a. RNF140, hDx-1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.3_DTX1 was a gift from J. Chad Brenner (Addgene plasmid # 124080 ; http://n2t.net/addgene:124080 ; RRID:Addgene_124080)