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Addgene

RCAS IkB K->A
(Plasmid #12408)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12408 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    RCAS
  • Backbone size w/o insert (bp) 11500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IkB alpha K->A
  • Alt name
    I kappa B alpha K->A mutant
  • Alt name
    I kappa B alpha
  • Species
    M. musculus (mouse)
  • Mutation
    Lysines 21, 22, and 67 are mutated to alanine. (The lysines are essential for targeting IkB for signal-induced degradation by the ubiquitin-proteasome system.)
  • Entrez Gene
    Nfkbia (a.k.a. Nfkbi)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS IkB K->A was a gift from Inder Verma (Addgene plasmid # 12408 ; http://n2t.net/addgene:12408 ; RRID:Addgene_12408)