RCAS IkB K->A
(Plasmid
#12408)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12408 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCAS
- Backbone size w/o insert (bp) 11500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIkB alpha K->A
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Alt nameI kappa B alpha K->A mutant
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Alt nameI kappa B alpha
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SpeciesM. musculus (mouse)
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MutationLysines 21, 22, and 67 are mutated to alanine. (The lysines are essential for targeting IkB for signal-induced degradation by the ubiquitin-proteasome system.)
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Entrez GeneNfkbia (a.k.a. Nfkbi)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS IkB K->A was a gift from Inder Verma (Addgene plasmid # 12408 ; http://n2t.net/addgene:12408 ; RRID:Addgene_12408)