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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
(Plasmid #124066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProEx-Htb
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cytATL-R232Q-sfGFP
  • Alt name
    X. laevis cytosolic Atlastin Fragment R232Q inactive mutant
  • Alt name
    Codon Optimized for E. Coli expression.
  • Species
    X. laevis (frog)
  • Mutation
    R232Q
  • Promoter trc
  • Tag / Fusion Protein
    • sfGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_Rev_primer
  • 3′ sequencing primer pBAD_Rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP was a gift from Tom Rapoport (Addgene plasmid # 124066 ; http://n2t.net/addgene:124066 ; RRID:Addgene_124066)

  • For your References section:

    Cooperation of the ER-shaping proteins atlastin, lunapark, and reticulons to generate a tubular membrane network. Wang S, Tukachinsky H, Romano FB, Rapoport TA. Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605. 10.7554/eLife.18605 PubMed 27619977
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