Skip to main content
Addgene

pGEX-4T-3-cytXlATL2-R232Q
(Plasmid #124064)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-4t-3
  • Backbone manufacturer
    Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cytXlATL2-R232Q
  • Alt name
    X. laevis cytosolic Atlastin fragment R232Q inactive mutant
  • Alt name
    Codon Optimized for E. Coli expression.
  • Species
    X. laevis (frog)
  • Promoter tac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX_5
  • 3′ sequencing primer pGEX_3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3-cytXlATL2-R232Q was a gift from Tom Rapoport (Addgene plasmid # 124064 ; http://n2t.net/addgene:124064 ; RRID:Addgene_124064)

  • For your References section:

    Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts. Wang S, Romano FB, Field CM, Mitchison TJ, Rapoport TA. J Cell Biol. 2013 Dec 9;203(5):801-14. doi: 10.1083/jcb.201308001. Epub 2013 Dec 2. 10.1083/jcb.201308001 PubMed 24297752
Related items: