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pGEX-4T-3-cytXlATL2
(Plasmid #124063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4t-3
  • Backbone manufacturer
    Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cytXIATL2
  • Alt name
    Cytosolic fragment of X laevis Atlastin. Lacks transmembrane segments and cytosolic c-terminal tail.
  • Alt name
    Codon Optimized for E. Coli expression.
  • Species
    X. laevis (frog)
  • Promoter tac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX_5
  • 3′ sequencing primer pGEX_3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3-cytXlATL2 was a gift from Tom Rapoport (Addgene plasmid # 124063 ; http://n2t.net/addgene:124063 ; RRID:Addgene_124063)

  • For your References section:

    Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts. Wang S, Romano FB, Field CM, Mitchison TJ, Rapoport TA. J Cell Biol. 2013 Dec 9;203(5):801-14. doi: 10.1083/jcb.201308001. Epub 2013 Dec 2. 10.1083/jcb.201308001 PubMed 24297752
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