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Addgene

HAP40 1-371
(Plasmid #124060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Arrowsmith lab (Addgene plasmid# 62304)
  • Backbone size w/o insert (bp) 6767
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HAP40
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1113
  • Entrez Gene
    F8A1 (a.k.a. DXS522E, F8A, HAP40)
  • Promoter polyhedrin
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGTATTTTACTGTTTTCG
  • 3′ sequencing primer TAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HAP40 1-371 was a gift from Cheryl Arrowsmith (Addgene plasmid # 124060 ; http://n2t.net/addgene:124060 ; RRID:Addgene_124060)

  • For your References section:

    Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH. J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. 10.1074/jbc.RA118.007204 PubMed 30842263
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