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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP73
- Backbone size w/o insert (bp) 2400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name135(T2AG3)
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Alt name800bp TTAGGG repeats
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Alt name138
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Insert Size (bp)800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (not destroyed)
- 3′ cloning site Sal I (destroyed during cloning)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSXneo 135(T2AG3) was a gift from Titia de Lange (Addgene plasmid # 12402 ; http://n2t.net/addgene:12402 ; RRID:Addgene_12402) -
For your References section:
Stringent sequence requirements for the formation of human telomeres. Hanish JP, Yanowitz JL, de Lange T. Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5. 10.1073/pnas.91.19.8861 PubMed 8090736