Top7 Y17L K39E K40E V48V K55E
(Plasmid
#12400)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet29 b(+)
- Backbone size w/o insert (bp) 5371
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTop7 Y19L K39E K40E V48V K55E
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Insert Size (bp)321
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MutationY19L K39E K40E V48V K55E
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Top7 Y17L K39E K40E V48V K55E was a gift from David Baker (Addgene plasmid # 12400 ; http://n2t.net/addgene:12400 ; RRID:Addgene_12400)