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Addgene

MTK234_055
(Plasmid #123920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MTK0_027
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
  • Species
    B. taurus (bovine)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MTK234_055 was a gift from Hana El-Samad (Addgene plasmid # 123920 ; http://n2t.net/addgene:123920 ; RRID:Addgene_123920)

  • For your References section:

    A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. 10.1021/acssynbio.9b00322 PubMed 31686495
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