UbC-TRE-mSOD2-tTS-IRES-EGFP
(Plasmid #12390)

• Depositing Lab: Zuoshang Xu
• Publication: Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Backbone

• Vector backbone: UbC-TRE-tTS-IRES-EGFP
• Backbone size w/o insert (bp): 7700
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSOD2 shRNA
• Alt name: Sod2
• Species: M. musculus (mouse)
• Insert Size (bp): 110
• Entrez Gene: Sod2 (a.k.a. MnSOD, Sod-2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • Xu-Cloning Protocol v2
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Additional Documents link above for cloning protocol, map, and annotated sequence from the author.

Note that there is a EcoRI and a KpnI site in the polylinker region. You will need to cut the plasmid with BamHI and religate to eliminate these sites before you could EcoRI and KpnI for cloning.