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Addgene

pAS2
(Plasmid #12389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pREP2
  • Backbone size w/o insert (bp) 8290
  • Vector type
    Yeast Expression
  • Selectable markers
    ura4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    green fluorescent protein
  • Alt name
    GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    1500
  • GenBank ID
    AAB02576

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS2 was a gift from Nick Rhind (Addgene plasmid # 12389 ; http://n2t.net/addgene:12389 ; RRID:Addgene_12389)

  • For your References section:

    A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe. Sigova A, Rhind N, Zamore PD. Genes Dev. 2004 Oct 1. 18(19):2359-67. 10.1101/gad.1218004 PubMed 15371329
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