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Addgene

pJLI-Sup35N-KDG6-C
(Plasmid #1238)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Backbone size w/o insert (bp) 6464
  • Vector type
    yeast integrative plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUP35
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1726
  • Mutation
    SUP35 middle region (aa124-253) was replaced with six repeats of the tripeptide "KDG"
  • GenBank ID
    AY553990
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJLI-Sup35N-KDG6-C was a gift from Susan Lindquist (Addgene plasmid # 1238 ; http://n2t.net/addgene:1238 ; RRID:Addgene_1238)

  • For your References section:

    Changes in the middle region of Sup35 profoundly alter the nature of epigenetic inheritance for the yeast prion [PSI+]. Liu JJ, Sondheimer N, Lindquist SL. Proc Natl Acad Sci U S A 2002 Dec 10;99 Suppl 4:16446-53. Epub 2002 Dec 02. 10.1073/pnas.252652099 PubMed 12461168
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