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Addgene

pFS118
(Plasmid #12378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIT28
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 2823
  • Vector type
    Yeast Expression
  • Selectable markers
    ura4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ura4, thymidine kinase
  • Species
    S. pombe (fission yeast); Herpes virus
  • Insert Size (bp)
    3750
  • GenBank ID
    CAA20910 X03764

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Paul Russell, TSRI Janet Leatherwood, SUNY Stonybrook
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFS118 was a gift from Nick Rhind (Addgene plasmid # 12378 ; http://n2t.net/addgene:12378 ; RRID:Addgene_12378)

  • For your References section:

    In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N. Methods. 2004 Jul . 33(3):213-9. 10.1016/j.ymeth.2003.11.016 PubMed 15157888
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