Syntaxin4-myc-myc-His
(Plasmid #12377)

• Depositing Lab: Thomas Weimbs
• Publication: Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Backbone

• Vector backbone: pcDNA4/TO/myc-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Syntaxin4
• Alt name: STX4
• Species: H. sapiens (human)
• Insert Size (bp): 891
• Mutation: Cloned in modified PCDNA4 vector (double myc)
• Entrez Gene: STX4 (a.k.a. STX4A, p35-2)
• Promoter: CMV⁄TO
• Tag / Fusion Protein:
  • 2xMyc-His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry