pBD-0001
(Plasmid #12373)

• Depositing Lab: Axel Brunger
• Publication: DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Backbone

• Vector backbone: pET-28a
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VCP
• Alt name: p97
• Species: M. musculus (mouse)
• Insert Size (bp): 2400
• Entrez Gene: Vcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Graham Warren at Yale, circa 2000
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry