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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCMV-LXSN
- Backbone size w/o insert (bp) 4500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegag/pol/env
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Insert Size (bp)8100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LXSN primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Packaging plasmid for pCL retroviruses. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371 ; http://n2t.net/addgene:12371 ; RRID:Addgene_12371) -
For your References section:
The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. Naviaux RK, Costanzi E, Haas M, Verma IM. J Virol. 1996 Aug . 70(8):5701-5. PubMed 8764092