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Addgene

pF420
(Plasmid #123659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1C3
  • Backbone manufacturer
    iGEM
  • Backbone size w/o insert (bp) 2070
  • Total vector size (bp) 8808
  • Modifications to backbone
    Additionally expresses TetR cassette BBa_K145201.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CofD
  • Alt name
    FbiA
  • Alt name
    EPPG:Fo phosphoenolpyruvyl transferase
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    909
  • GenBank ID
    MM_RS09715
  • Promoter BBa_R0040

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer GTTCACCACGTTTACCAATC
  • 3′ sequencing primer AACCAGCACATCACCGTCAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    FbiB
  • Alt name
    CofB
  • Alt name
    F420-0:γ-glutamate ligase
  • Species
    Mycobacterium smegmatis
  • Insert Size (bp)
    1362
  • GenBank ID
    MSMEG_1892
  • Promoter BBa_R0040

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer AACCAGCACATCACCGTCAC
  • 3′ sequencing primer ACCTGTTTTGGCTGGGCACC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    FbiD
  • Alt name
    CofC
  • Alt name
    phosphoenolpyruvate guanylyltransferase
  • Species
    Mycobacterium smegmatis
  • Insert Size (bp)
    663
  • GenBank ID
    MSMEG_2392
  • Promoter BBa_R0040

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer TGCAGCAAGTGATGGTAGTG
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    FbiC
  • Alt name
    CofGH
  • Alt name
    FO Synthase
  • Species
    Mycobacterium smegmatis
  • Insert Size (bp)
    2622
  • GenBank ID
    MSMEG_5126
  • Promoter BBa_R0040

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer TAAGGAGCCCCCATAATGCG
  • 3′ sequencing primer ATGGTCATGTTGATCAGCCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full set of sequencing primers:

U4965CK070-6-SEQ1R AACCAGCACATCACCGTCAC
U4965CK070-6-SEQ2R CGGTCAGGGCTTCACCAACT
U4965CK070-6-SEQ3 ACCTGTTTTGGCTGGGCACC
U4965CK070-6-SEQ4 TGCAGCAAGTGATGGTAGTG
U4965CK070-6-SEQ5 TAAGGAGCCCCCATAATGCG
U4965CK070-6-SEQ6N GAGTTGGAGCGAACTGAGTC
U4965CK070-6-SEQ7 ACCGCCTAATCTGGTTAGCC
U4965CK070-6-SEQ8 CCTGGTGGTTGTAGTGATGA
U4965CK070-6-SEQ9 ATGGTCATGTTGATCAGCCT
U4965CK070-6-SEQ0.5r GTTCACCACGTTTACCAATC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF420 was a gift from Colin Jackson (Addgene plasmid # 123659 ; http://n2t.net/addgene:123659 ; RRID:Addgene_123659)

  • For your References section:

    A revised biosynthetic pathway for the cofactor F420 in prokaryotes. Bashiri G, Antoney J, Jirgis ENM, Shah MV, Ney B, Copp J, Stuteley SM, Sreebhavan S, Palmer B, Middleditch M, Tokuriki N, Greening C, Scott C, Baker EN, Jackson CJ. Nat Commun. 2019 Apr 5;10(1):1558. doi: 10.1038/s41467-019-09534-x. 10.1038/s41467-019-09534-x PubMed 30952857
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