pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4
(Plasmid
#123658)
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Purpose(Empty Backbone) DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
- Backbone size (bp) 8206
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Vector typeMammalian Expression, Lentiviral, CRISPR, Synthetic Biology
- Promoter mU6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4 was a gift from Stanley Qi (Addgene plasmid # 123658 ; http://n2t.net/addgene:123658 ; RRID:Addgene_123658) -
For your References section:
Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. Nakamura M, Srinivasan P, Chavez M, Carter MA, Dominguez AA, La Russa M, Lau MB, Abbott TR, Xu X, Zhao D, Gao Y, Kipniss NH, Smolke CD, Bondy-Denomy J, Qi LS. Nat Commun. 2019 Jan 14;10(1):194. doi: 10.1038/s41467-018-08158-x. 10.1038/s41467-018-08158-x [pii] PubMed 30643127