Cas9-PCV
(Plasmid #123644)

• Purpose: Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli
• Depositing Lab: Wendy Gordon
• Publication: Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018.

Backbone

• Vector backbone: pTD68
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: PCV2
• Alt name: porcine circovirus2 Rep domain
• Mutation: 2-116
• Entrez Gene: ORF1 (a.k.a. PCV2_gp1)
• Tags / Fusion Proteins:
  • SUMO (N terminal on backbone)
  • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Gene/Insert 2

• Gene/Insert name: Cas9
• Alt name: S. pyogenes Cas9

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry