PCV-Cas9
(Plasmid #123643)

• Purpose: Expresses Cas9 with an N-terminal HUH-tag called PCV2
• Depositing Lab: Wendy Gordon
• Publication: Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018.

Backbone

• Vector backbone: pTD68
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: PCV2
• Alt name: porcine circovirus2 Rep domain
• Mutation: 2-116
• Entrez Gene: ORF1 (a.k.a. PCV2_gp1)
• Tags / Fusion Proteins:
  • His6 + SUMO (N terminal on backbone)
  • (EAAAK)8 linker (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Cas9
• Alt name: S. pyogenes Cas9

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Cas9- from Addgene #62731, PCV2 was synthetic gene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry