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Addgene

PCV-Cas9
(Plasmid #123643)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123643 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTD68
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PCV2
  • Alt name
    porcine circovirus2 Rep domain
  • Mutation
    2-116
  • Entrez Gene
    ORF1 (a.k.a. PCV2_gp1)
  • Tags / Fusion Proteins
    • His6 + SUMO (N terminal on backbone)
    • (EAAAK)8 linker (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Cas9
  • Alt name
    S. pyogenes Cas9

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Cas9- from Addgene #62731, PCV2 was synthetic gene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCV-Cas9 was a gift from Wendy Gordon (Addgene plasmid # 123643 ; http://n2t.net/addgene:123643 ; RRID:Addgene_123643)
  • For your References section:

    Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template. Aird EJ, Lovendahl KN, St Martin A, Harris RS, Gordon WR. Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018. 10.1038/s42003-018-0054-2 PubMed 30271937