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PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTD68
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePCV2
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Alt nameporcine circovirus2 Rep domain
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Mutation2-116
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Entrez GeneORF1 (a.k.a. PCV2_gp1)
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Tags
/ Fusion Proteins
- His6 + SUMO (N terminal on backbone)
- (EAAAK)8 linker (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameCas9
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Alt nameS. pyogenes Cas9
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byCas9- from Addgene #62731, PCV2 was synthetic gene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCV-Cas9 was a gift from Wendy Gordon (Addgene plasmid # 123643 ; http://n2t.net/addgene:123643 ; RRID:Addgene_123643) -
For your References section:
Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template. Aird EJ, Lovendahl KN, St Martin A, Harris RS, Gordon WR. Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018. 10.1038/s42003-018-0054-2 PubMed 30271937