pUC19-U6-crRNA4n96
(Plasmid
#123640)
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PurposeMammalian expression, Genome editing, gRNA scaffold
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 123640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19, BsaI site mutated
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecrRNA scaffold 4n96
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Alt namecrRNA4n96
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gRNA/shRNA sequenceATTTCTACTATgGTAGAT
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-U6-crRNA4n96 was a gift from Wei Li (Addgene plasmid # 123640 ; http://n2t.net/addgene:123640 ; RRID:Addgene_123640) -
For your References section:
Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q, Li W. Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8. 10.1186/s13059-019-1620-8 PubMed 30717767
