Skip to main content
Addgene

pUC19-U6-crRNA4n96
(Plasmid #123640)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123640 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19, BsaI site mutated
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crRNA scaffold 4n96
  • Alt name
    crRNA4n96
  • gRNA/shRNA sequence
    ATTTCTACTATgGTAGAT
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-U6-crRNA4n96 was a gift from Wei Li (Addgene plasmid # 123640 ; http://n2t.net/addgene:123640 ; RRID:Addgene_123640)

  • For your References section:

    Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q, Li W. Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8. 10.1186/s13059-019-1620-8 PubMed 30717767
Related items:
Commonly requested with: