pBXPC3H-Syb37
(Plasmid
#123627)
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PurposeExpresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 123627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBXPC3H
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyb37
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SpeciesSynthetic
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Insert Size (bp)360
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Tags
/ Fusion Proteins
- PelB (N terminal on insert)
- 3C-His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBXPC3H-Syb37 was a gift from Simon Newstead (Addgene plasmid # 123627 ; http://n2t.net/addgene:123627 ; RRID:Addgene_123627) -
For your References section:
Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor. Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S. Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. 10.1126/science.aaw2859 PubMed 30846601
