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Addgene

pDDGFP-Leu2d-GgKDELR2 E127Q
(Plasmid #123623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDDGFP-Leu2d
  • Backbone manufacturer
    Plasmid #58352
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDELR2
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    696
  • Mutation
    Glu127Gln
  • Entrez Gene
    KDELR2
  • Tag / Fusion Protein
    • TEV-GFP-His (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDDGFP-Leu2d-GgKDELR2 E127Q was a gift from Simon Newstead (Addgene plasmid # 123623 ; http://n2t.net/addgene:123623 ; RRID:Addgene_123623)

  • For your References section:

    Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor. Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S. Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. 10.1126/science.aaw2859 PubMed 30846601
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