pDDGFP-Leu2d-GgKDELR2 R47A
(Plasmid
#123620)
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PurposeExpresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDDGFP-Leu2d
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Backbone manufacturerPlasmid #58352
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKDELR2
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SpeciesG. gallus (chicken)
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Insert Size (bp)696
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MutationArg47Ala
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Entrez GeneKDELR2
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Tag
/ Fusion Protein
- TEV-GFP-His (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDDGFP-Leu2d-GgKDELR2 R47A was a gift from Simon Newstead (Addgene plasmid # 123620 ; http://n2t.net/addgene:123620 ; RRID:Addgene_123620) -
For your References section:
Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor. Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S. Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. 10.1126/science.aaw2859 PubMed 30846601