BE3(E63Q)-P2A-EGFP (pRZ189)
(Plasmid
#123613)
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PurposeCAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCAG
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Backbone manufacturerpSQT817 (Addgene #53373)
- Backbone size w/o insert (bp) 4843
- Total vector size (bp) 10768
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBE3(E63Q)-P2A-EGFP
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Alt namerAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP
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SpeciesR. norvegicus (rat), Synthetic; Streptococcus pyogenes, Bacillus subtilis bacteriophage PBS1
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Insert Size (bp)5925
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MutationE63Q in rAPOBEC1, D10A in SpCas9
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GenBank ID
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTCTGCTAACCATGTTCATGCC
- 3′ sequencing primer CAGAGGGAAAAAGATCTCAGTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLiu Lab (BE3, Addgene #73021) and Benjamin Kleinstiver (BPK4335)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BE3(E63Q)-P2A-EGFP (pRZ189) was a gift from Keith Joung (Addgene plasmid # 123613 ; http://n2t.net/addgene:123613 ; RRID:Addgene_123613) -
For your References section:
Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. 10.1038/s41586-019-1161-z PubMed 30995674