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Addgene

pBD-0039
(Plasmid #12359)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12359 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VCP F552A
  • Alt name
    p97
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    I206V, F552A
  • Entrez Gene
    Vcp
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Graham Warren at Yale, circa 2000

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a I206V mutation in the VCP sequence when compared to GenBank reference NP_033529.3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBD-0039 was a gift from Axel Brunger (Addgene plasmid # 12359 ; http://n2t.net/addgene:12359 ; RRID:Addgene_12359)

  • For your References section:

    Central pore residues mediate the p97/VCP activity required for ERAD. DeLaBarre B, Christianson JC, Kopito RR, Brunger AT. Mol Cell. 2006 May 19. 22(4):451-62. 10.1016/j.molcel.2006.03.036 PubMed 16713576
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