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Addgene

pMK-N. tabacum trnI/trnA
(Plasmid #123579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMK-RQ
  • Vector type
    Plant Expression ; Chloroplast transformation vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N. tabacum trnI/trnA integration site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. This is a chloroplast transformation vector with the indicated homologous arms. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK-N. tabacum trnI/trnA was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123579 ; http://n2t.net/addgene:123579 ; RRID:Addgene_123579)

  • For your References section:

    MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266
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