pBD-0013
(Plasmid
#12346)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVCP R313A
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Alt namep97
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SpeciesM. musculus (mouse)
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Insert Size (bp)2400
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MutationR313A
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Entrez GeneVcp
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGraham Warren at Yale, circa 2000
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBD-0013 was a gift from Axel Brunger (Addgene plasmid # 12346 ; http://n2t.net/addgene:12346 ; RRID:Addgene_12346) -
For your References section:
Central pore residues mediate the p97/VCP activity required for ERAD. DeLaBarre B, Christianson JC, Kopito RR, Brunger AT. Mol Cell. 2006 May 19. 22(4):451-62. 10.1016/j.molcel.2006.03.036 PubMed 16713576