pCLXSN
(Plasmid #12343)

• Purpose: (Empty Backbone)
• Depositing Lab: Inder Verma
• Publication: Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

Backbone

• Vector backbone: pCLXSN
• Backbone manufacturer: Verma Lab
• Backbone size (bp): 7807
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LXSN primer

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 3.1-kb fragment of LXSN, spanning from the SacI site in the 5'LTR near the U3-R junction to the AccI site just beyond the 3'LTR, was cloned into the 4.5-kb CMV expression construct, CMX-low, at a corresponding SacI site within the CMV immediate early enhancer-promoter.

NOTE: The depositor map indicated an EcoRI site present in this plasmid, but that site is ablated by a small insert.

Note that the full sequence and subsequent map are assembled and have not been verified by full sequencing. Please take this into account when submitting this plasmid to diagnostic digests.