pCL-MFG-BRCA1
(Plasmid #12341)

• Depositing Lab: Inder Verma
• Publication: Ruffner et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43.

Backbone

• Vector backbone: pCLMFG
• Backbone manufacturer: Verma Lab
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (partial digest) (not destroyed)
• 3′ cloning site: XbaI/BamHI (destroyed during cloning)
• 5′ sequencing primer: LXSN primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector.

Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.