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Addgene

pCSC-SP-PW-NepX (aka: pBOB-NEPX)
(Plasmid #12340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12340 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBOB
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEP-X
  • Alt name
    neprilysin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2250
  • Mutation
    E585V (inactive mutant)
  • Entrez Gene
    MME (a.k.a. CALLA, CD10, CMT2T, NEP, SCA43, SFE)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence and map shown are for the wild-type Nep plasmid. The NepX plasmid contains an inactivating E585V mutation in the Nep gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSC-SP-PW-NepX (aka: pBOB-NEPX) was a gift from Inder Verma (Addgene plasmid # 12340 ; http://n2t.net/addgene:12340 ; RRID:Addgene_12340)

  • For your References section:

    Neprilysin regulates amyloid Beta peptide levels. Marr RA, Guan H, Rockenstein E, Kindy M, Gage FH, Verma I, Masliah E, Hersh LB. J Mol Neurosci. 2004 . 22(1-2):5-11. 10.1385/JMN:22:1-2:5 PubMed 14742905
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