pcDNA3-AktAR-Kras
(Plasmid #123357)

• Purpose: FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.
• Depositing Lab: Jin Zhang
• Publication: Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73. doi: 10.1091/mbc.E08-05-0449. Epub 2008 Aug 13.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5471
• Total vector size (bp): 7526
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AktAR-Kras
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 2055
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cerluean (N terminal on insert)
  • cpVenus[E172] (C terminal on insert)
  • C-terminal targeting sequence from Kras (C terminal on insert)
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-AktAR-Kras

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry