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pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER
(Plasmid #123354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFPC1
  • Backbone size w/o insert (bp) 3933
  • Total vector size (bp) 6238
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
  • Species
    Synthetic
  • Insert Size (bp)
    2305
  • Promoter CMV
  • Tags / Fusion Proteins
    • cp-moxCerulean3[E173] (N terminal on insert)
    • moxCerulean3 (C terminal on insert)
    • C-terminal ER retention signal (KDEL) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER was a gift from Jin Zhang (Addgene plasmid # 123354 ; http://n2t.net/addgene:123354 ; RRID:Addgene_123354)

  • For your References section:

    Single-color, ratiometric biosensors for detecting signaling activities in live cells. Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE, McFarland JJ, Mauban JR, Ward CW, Rizzo MA, Zhang J. eLife. 2018 Jul 3;7. pii: 35458. doi: 10.7554/eLife.35458 10.7554/eLife.35458 PubMed 29968564
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