pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR
(Plasmid
#123337)
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PurposePlasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5415
- Total vector size (bp) 7431
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn-Venus-cpVenus-FLARE-AKAR
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)2016
- Promoter CMV
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Tags
/ Fusion Proteins
- Venus (N terminal on insert)
- cpVenus[E172] (C terminal on insert)
- N-terminal targeting sequence from Lyn kinase (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR was a gift from Jin Zhang (Addgene plasmid # 123337 ; http://n2t.net/addgene:123337 ; RRID:Addgene_123337) -
For your References section:
Single-color, ratiometric biosensors for detecting signaling activities in live cells. Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE, McFarland JJ, Mauban JR, Ward CW, Rizzo MA, Zhang J. Elife. 2018 Jul 3;7. pii: 35458 10.7554/eLife.35458 PubMed 29968564