Skip to main content
Addgene

pCW-IRF4-Puro
(Plasmid #123323)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCW-MCS-Puro
  • Total vector size (bp) 9038
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1356
  • Mutation
    several silent mutations to disrupt sgRNA targeting
  • Entrez Gene
    IRF4 (a.k.a. LSIRF, MUM1, NF-EM5, SHEP8)
  • Promoter Doxycycline inducible minimal CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-IRF4-Puro was a gift from Eva Gottwein (Addgene plasmid # 123323 ; http://n2t.net/addgene:123323 ; RRID:Addgene_123323)

  • For your References section:

    CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. 10.1182/blood-2018-01-828418 PubMed 29954751
Related items:
Commonly requested with: