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Addgene

pLKO.1 Blast NT shRNA
(Plasmid #123306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1 Puro
  • Modifications to backbone
    Puro resistance replaced with Blasticidin resistance
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Non-targeting shRNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 Blast NT shRNA was a gift from Giovanni Tonon (Addgene plasmid # 123306 ; http://n2t.net/addgene:123306 ; RRID:Addgene_123306)

  • For your References section:

    Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G. EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. 10.15252/embj.201899179 PubMed 30373810
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