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Addgene

pLenti C-MYC-DDK Puro PNRC1
(Plasmid #123300)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123300 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti-C-MYC-DDK Puro
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PNRC1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PNRC1 (a.k.a. B4-2, PNAS-145, PROL2, PRR2)
  • Tag / Fusion Protein
    • MYC-FLAG (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti C-MYC-DDK Puro PNRC1 was a gift from Giovanni Tonon (Addgene plasmid # 123300 ; http://n2t.net/addgene:123300 ; RRID:Addgene_123300)
  • For your References section:

    Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G. EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. 10.15252/embj.201899179 PubMed 30373810