pLenti C-MYC-DDK Puro PNRC1
(Plasmid
#123300)
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PurposeMammalian expression of PNRC1 C-MYC-FLAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-C-MYC-DDK Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePNRC1
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SpeciesH. sapiens (human)
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Entrez GenePNRC1 (a.k.a. B4-2, PNAS-145, PROL2, PRR2)
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Tag
/ Fusion Protein
- MYC-FLAG (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti C-MYC-DDK Puro PNRC1 was a gift from Giovanni Tonon (Addgene plasmid # 123300 ; http://n2t.net/addgene:123300 ; RRID:Addgene_123300) -
For your References section:
Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G. EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. 10.15252/embj.201899179 PubMed 30373810